Investigation of minerals, testosterone, and transaminases in the semen and serum of fertile and infertile men belongs to different age groups

The present study was aimed to assess the potential of infertility to induce the adverse effects with reference to testosterone, Triiodothyronine (T3), Thyroxine (T4), Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), zinc, copper and iron. All the samples were divided into four groups according to age and disorder (Group 1, 10 infertile men of 25 40 years; Group 2, 10 fertile men of 25 40 years; Group 3, 10 infertile men of 41 60 years and Group 4, 10 fertile men of 41 60 years). Semen and blood samples were analyzed by atomic absorption spectrophotometry to determine minerals while, Testosterone, T3 and T4 were determined by enzyme immunoassay kits. ALT and AST were determined using standard kit assay method. The levels of testosterone and T3 and AST in the fertile semen of 41 60 years age group were increased significantly (P ≤ 0.001) as compared to that of fertile semen of 25 40 years age group. While, the level of T4 in the fertile semen of 41 60 years age group was decreased significantly (P ≤ 0.001) as compared to that of fertile semen of 25 40 years age group. In case of fertile serum, only the level of testosterone was significantly decreased (P ≤ 0.05) in the 41 60 years age group as compared to 25 40 years age group. The levels of testosterone and Cu in the infertile serum of 41 60 years age group were decreased significantly (P ≤ 0.001). While, the levels of T3, T4, ALT and Fe in the infertile serum of 41 60 years age group were increased significantly (P ≤ 0.05) as compared to that of infertile serum of 25 40 years age group.